Notes

Slide Show

Outline

1	Global fitting
2	What is Global Model-Fitting?
3	"Prism 4 would not have..." Prism 4 would not have shared parameters if not for the help, and repeated nagging, of Arthur Christopoulos Dept. of Pharmacology, University of Melbourne … who also created initial drafts of many of these slides.
4	Global Model-Fitting
5	Incomplete Data Sets
6	Concentration-Response Curves
7	Concentration-Response Curves
8	Concentration-Response Curves
9	Concentration-Response Curves
10	Concentration-Response Curves
11	Concentration-Response Curves
12	Concentration-Response Curves
13	Comparing Models
14	F-Test (Extra Sum-Of-Squares Test)
15	F-Test: Standard Example. Comparing fit of two models to one data set
16	Akaike’s Information Criterion Comparing fit of two models to one data set
17	F-Test, or AIC, after Global Fitting
18	Comparing Curves
19	Comparing Curves
20	Comparing Curves
21	How to answer? Rephrase the question to compare models, with our without sharing.
22	Comparing Curves
23	Comparing Curves
24	Comparing Curves
25	Comparing Curves
26	Comparing Curves with F test
27	Comparing Curves with AICc
28	Comparing Parameters
29	Comparing Parameters
30	Comparing Parameters
31	Comparing Parameters
32	Comparing Parameters
33	Comparing Parameters, with F test
34	Comparing Parameters, with AICc
35	Fitting Models where the parameters you care about come from two or more data sets (not one).
36	Example 1: Radioligand Saturation Binding
37	Example 1: Radioligand Saturation Binding
38	Example 1: Radioligand Saturation Binding
39	Example 1: Radioligand Saturation Binding
40	Example 1: Radioligand Saturation Binding
41	Example 1: Radioligand Saturation Binding
42	Example 1: Radioligand Saturation Binding
43	Example 1: Radioligand Saturation Binding
44	Example 1: Radioligand Saturation Binding
45	Example 1: Radioligand Saturation Binding
46	Example 1: Radioligand Saturation Binding
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49	Example 2: Homologous Competition Binding
50	Homologous competition binding. If you fit only one curve, results are usually not clear.
51	Solution. Perform Homologous Competition Binding Experiments with Two Different Concentrations of Ligand
52	Example 2: Homologous Competition Binding
53	Example 2: Homologous Competition Binding
54	In Prism 4
55	Example 2: Homologous Competition Binding
56	Example 2: Homologous Competition Binding
57	Example 3: Agonist-Antagonist C/R Curve Analysis
58	Example 3: Agonist-Antagonist C/R Curve Analysis
59	Example 3: Agonist-Antagonist C/R Curve Analysis
60	Example 3: Agonist-Antagonist C/R Curve Analysis
61	Example 3: Agonist-Antagonist C/R Curve Analysis
62	Example 3: Agonist-Antagonist C/R Curve Analysis
63	Example 3: Agonist-Antagonist C/R Curve Analysis
64	How to set constants with different values for each data set in Prism 4
65	Example 3: Agonist-Antagonist C/R Curve Analysis
66	Example 3: Agonist-Antagonist C/R Curve Analysis
67	Example 3: Agonist-Antagonist C/R Curve Analysis
68	Final Example – Kinetic Binding Curves
69	Final Example – Kinetic Binding Curves
70	Final Example – Kinetic Binding Curves
71	Final Example – Kinetic Binding Curves
72	Final Example – Kinetic Binding Curves
73	Global fitting Better answers. Easier!! (at least with Prism4)
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