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This guide is for an old version of Prism. Browse the latest version or update Prism

Groups are defined by rows and columns

Prism organizes data for three-way ANOVA differently than do most other programs. Here is an example of data entry:

Note that Prism does not use grouping variables. Instead, you only enter the outcome variable and designate treatments or groups by choice of rows and columns.

Rows 1 and 2 represent two levels of one factor (male vs. female) in the example. You can use more than two rows if this factor has more than three levels.

Data set columns A and B vs. C and D represent the second factor, Control vs. Treated in the example.

Data set columns A and C vs. B and D represent the third factor, low vs. high dose in the example.

Only data in the first four data set columns (A-D) are considered in three-way ANOVA.

The example shows two subcolumns, for duplicate data. But you can choose any number of subcolumns you want (up to 256).

Setting up the data table

From the Welcome (or New Data Table and Graph) dialog, choose the Grouped tab.

Entering raw data

If you are not ready to enter your own data, choose to use sample data and choose the three-way ANOVA sample data sets.

If you plan to enter your own data, create a table with the right number subcolumns to hold your replicated measurements.

Entering averaged data

If you have already averaged your replicates in another program, you can choose to enter and plot the mean and SD (or SEM) and n. If your data has more than 256 replicates, this is the only way to enter data into Prism for three-way ANOVA.

Running three-way ANOVA

1.From the data table, click on the toolbar.

2.Choose three-way ANOVA from the list of grouped analyses.

3. On the first tab (Experimental Design), name the grouping variables.

4. On the second (Multiple Comparisons) and third (Options) tab, choose multiple comparisons.

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