

The graph above shows three ways to plot the sample data for twoway ANOVA.
The graphs on the left and middle interleave the data sets. This is set on the second tab of the Format Graphs dialog. In this case, the data sets are defined by the figure legend, and the groups (rows) are defined by the labels on the X axis.
The graph on the right has the data sets grouped. In this graph, the labels on the X axis show the row title  one per bar. Use the "number format" choice in the Format Axes dialog to change this to Column titles  one per set of bars. With this choice, there wouldn't be much point in also having the legend shown in the box, and you would need to define the side by side bars ("serum starved" vs "normal culture" for this example) in the figure legend.
The graph on the left has the appearance set as a column dot plot. The other two graphs have the appearance set as bars with error bars plotted from the mean and SD. I prefer the column dot plot as it shows all the data, without taking up more space and without being harder to interpret. If you have a huge number of replicates, use a violin plot instead.
Don't forget to include in the figure legend whether the error bars are SD or SEM or something different.