

Prism can test for linear trend as part of the followup testing after oneway (but not two or threeway) ANOVA. It is a choice on the Multiple Comparisons tab of the parameters dialog for oneway ANOVA.
This test makes sense when the columns represent ordered and equally spaced (or nearly so) groups. For example, the columns might represent age groups, or doses or times. The test for linear trend asks whether the column means increase (or decrease) systematically as the columns go from left to right. Prism does the calculations setting column A to 1, column B to 2 and so on. You cannot assign other values to the columns. If you enter values into the column titles, they will be ignored.
Alternative names are testing for a linear contrast, posttest for trend, and test for linear trend.
Note that ANOVA calculations (except for this trend test) completely ignore the order of the columns. You could randomly scramble the column order, and get exactly the same ANOVA results. So if the columns represent time points or concentrations or anything that can be quantified, think hard about whether ANOVA is the best way to analyze your data. Rather than do ANOVA followed by a test for linear trend, you may want to fit the data with linear (or nonlinear) regression.
After you've entered your data for ANOVA, you'll need to rearrange (transpose) the data if you want to analyze the same data with regression.
1.From the column table used for ANOVA, click Analyze and choose the Transpose analysis.
2.Select that the results should be an XY results table. Choose sequential X values values if the columns are equallyd and you want to match the results o the test for trend. Enter your own X values if the values associated with each column are not equally spaced.
3.From the completed XY table, click Analyze and choose linear regression.