Our goal with Prism has always been to make basic biostatistics very accessible and easy. Two-way ANOVA is pushing the limits of "basic biostatistics". Multiple comparisons after two-way ANOVA stretch this definition even more. If you haven't taken the time to really understand two-way ANOVA, it is quite easy to be mislead by the results. Beware!

•Two-way ANOVA is not a topic that is easy to master. In addition to reading textbooks, also consider getting help.from someone with more experience.

•Before getting lost in the many choices for multiple comparisons, first articulate clearly the scientific goals of the study. Don't articulate your goals in terms of ANOVA (looking for interactions). Figure out what you really want to know. Then figure out the best statistical approach to getting the answer.

•In Prism, the two-way ANOVA analysis can be used when, as the name suggests, there are two factors. None, one or both of the factors can be repeated measures.