GraphPad Curve Fitting Guide

Key concepts: Deming regression

Key concepts: Deming regression

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Key concepts: Deming regression

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Standard linear regression assumes that you know the X values perfectly, and all the uncertainty is in Y. It minimizes the sum of squares of the vertical distance of the points from the line.

If both X and Y variables are subject to error, fit linear regression using a method known as Deming, or Model II, regression.

If your goal is to compare two analysis methods, consider using a Bland-Altman plot instead.