GraphPad Prism 8 User Guide

Symbol shape and border

Symbol shape and border

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Symbol shape and border

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You can plot symbols on Column, Grouped, Contingency, and XY graphs.

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Notes:

You will only have choices of border color and thickness when you choose an open symbol (as shown above).

The standard set of symbols will probably have what you want:
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If you click "More", you can choose any character from any font to use instead of a symbol.