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The figure above shows a saturation binding equation fit to specific binding data. But all the concentrations are relatively low (compared to the Kd of binding) ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Many dose-response curves follow a familiar sigmoidal shape. These curves are often defined by four parameters: Top, Bottom, Hill slope, and the EC50 (or IC50).

https://www.graphpad.com/guides/prism/latest/curve-fitting/

The standard dose-response curve is sometimes called the four-parameter logistic equation. It fits four parameters: the bottom and top plateaus of the curve, ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

What are residuals? Unweighted fits A residual is the distance of a point from the curve. Least-squares regression works to minimize the sum of the squares ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Browse the Statistics and Regression guides This Prism Guide is only one of three guides. Check out the other two. Both do more than explain how to use ...

https://www.graphpad.com/guides/prism/latest/user-guide/