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Prism lets you customize bar graphs, to combine interleaving and stacking. To make this kind of graph, download the Prism file.

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Enter the data with the technical replicates stacked. In the example, you tested between 4 and 6 students in each room and these are stacked in each subcolumn.

https://www.graphpad.com/guides/prism/latest/statistics/

... stacked, interleave, grouped, or superimposed. ... If your data table has only one row, Prism will place the column titles, rather than row titles, under each bar ...

https://www.graphpad.com/guides/prism/latest/user-guide/

Click Analyze, look in the list of Column analyses, and choose Analyze a stack of P values. Choose one of two general approaches: Control the False Discovery ...

https://www.graphpad.com/guides/prism/latest/statistics/

Place computed unknown values into the X column, with replicate values stacked ... In this case, Prism creates two tabs of results. The interpolation is the same ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Prism 8 introduces a new kind of data table for nested data where values stacked in each subcolumn are related, and lets you graph these sensibly.

https://www.graphpad.com/guides/prism/latest/user-guide/

But when you identify outliers in a stack of column data, Prism does not automatically identify those outliers on the graph. ... On the column table, each group ...

GraphPad FAQs

AND Each row represents a different time point, so matched values are stacked into a subcolumn. Disabled multiple comparisons test: Compare column means (main ...

GraphPad FAQs

Enter replicate values stacked into columns. Enter the data for each group into a separate column. The two groups do not have be the same size (it's OK to ...

https://www.graphpad.com/guides/prism/latest/statistics/

Prism 5 and earlier versions doesn't let you make a graph with bars or columns and also show the individual data points as a scatter plot. The workaround is to ...

GraphPad FAQs