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You have a choice, in the Confidence tab, to either mark selected parameters as "unstable", to mark entire fits as "ambiguous", or neither.

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Fit the ratio of two IC50 values directly. From the results in step 8, you can compute what you want to know -- the ratio of the two EC50 values. But Prism ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

This is not the same as Vmax,which would require a maximal concentration of substrate. Ki is the inhibition constant, expressed in the same units as X. The IC50 ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

The only difference is that the inhibitory equations fit the IC50 ("I" for inhibition) while the stimulation equations fit the EC50 ("E" for effective). If ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

It fits four parameters: the bottom and top plateaus of the curve, the EC50 (or IC50), and the slope factor (Hill slope). This curve is symmetrical around ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Are you fitting the relative IC50 but expecting an absolute IC50, and so are surprised by the results. © 1995-2025 GraphPad Software, LLC. All rights ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Additional output (legacy for old Prism versions). The options here (dose ratios, and Ki from IC50) are available only to be compatible with earlier versions of ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Equation: Absolute IC50. Receptor binding - Key concepts; Receptor binding - Saturation binding; Receptor binding - Competitive binding; Receptor binding ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

It fits four parameters: the bottom and top plateaus of the curve, the EC50 (or IC50), and the slope factor (Hill slope). This curve is symmetrical around ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Relative vs absolute IC50; Fitting the absolute IC50; Incomplete dose-respone curves; Troubleshooting fits of dose-response curves. Dose-response - Stimulation ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/