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Ki is the inhibition constant, expressed in the same units as X. The IC50 is not the same as the Ki (Kusmic). Instead, IC50 = Et/2 + Ki. Reference.

https://www.graphpad.com/guides/prism/latest/curve-fitting/

You have a choice, in the Confidence tab, to either mark selected parameters as "unstable", to mark entire fits as "ambiguous", or neither.

https://www.graphpad.com/guides/prism/latest/curve-fitting/

The only difference is that the inhibitory equations fit the IC50 ("I" for inhibition) while the stimulation equations fit the EC50 ("E" for effective). If ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Equation: Absolute IC50. Receptor binding - Key concepts; Receptor binding - Saturation binding; Receptor binding - Competitive binding; Receptor binding ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Instead you enter the concentration of radioligand and its Kd as constants, and Prism directly fits the Ki of your cold compound. If you want to know the IC50, ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Fit the ratio of two IC50 values directly. From the results in step 8, you can compute what you want to know -- the ratio of the two EC50 values. But Prism ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

It fits four parameters: the bottom and top plateaus of the curve, the EC50 (or IC50), and the slope factor (Hill slope). This curve is symmetrical around ...

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The term IC50 is used more frequently ("E" stands for effective; "I" stands for inhibitory). Usually the Y values are total binding. If you enter specific ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Are you fitting the relative IC50 but expecting an absolute IC50, and so are surprised by the results. © 1995-2026 GraphPad Software, LLC. All rights ...

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Additional output (legacy for old Prism versions). The options here (dose ratios, and Ki from IC50) are available only to be compatible with earlier versions of ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/