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Instead you enter the concentration of radioligand and its Kd as constants, and Prism directly fits the Ki of your cold compound. If you want to know the IC50, ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Fit the ratio of two IC50 values directly. From the results in step 8, you can compute what you want to know -- the ratio of the two EC50 values. But Prism ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

This calculator computes EC 80 or EC 90 (etc.) from EC 50. How? 1. Enter EC 50 I will enter: EC 50 as concentration. For 1nM, enter '1e-9'.

https://www.graphpad.com/quickcalcs/ecanything1/

This is not the same as Vmax,which would require a maximal concentration of substrate. Ki is the inhibition constant, expressed in the same units as X. The IC50 ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

https://graphpad.com/guides/prism/7/curve-fitting/

It fits four parameters: the bottom and top plateaus of the curve, the EC50 (or IC50), and the slope factor (Hill slope). This curve is symmetrical around ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

The only difference is that the inhibitory equations fit the IC50 ("I" for inhibition) while the stimulation equations fit the EC50 ("E" for effective). If ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Make sure you pick the form of the equation to match the data you are analyzing. The uncertainty in the value of the EC50 or IC50 is usually quite asymmetrical.

https://www.graphpad.com/guides/prism/latest/curve-fitting/

It fits four parameters: the bottom and top plateaus of the curve, the EC50 (or IC50), and the slope factor (Hill slope). This curve is symmetrical around ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Additional output (legacy for old Prism versions). The options here (dose ratios, and Ki from IC50) are available only to be compatible with earlier versions of ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/