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## Course Content

Key Concepts of Prism Data Tables

When to Use the Column Data Table

When to Use the Multiple Variables Data Table

When to Use Contingency, Survival, or Parts of a Whole Data Tables

How to Perform a T Test in Prism

Learn to Perform Repeated Measures One-way ANOVA in Prism

Learn to Perform a Three-way ANOVA in Prism

How to Perform a Kaplan-Meier Survival Analysis in Prism

Principal Component Analysis (PCA) in Prism

How Principal Component Analysis (PCA) Works

Understanding Your Principal Component Analysis Results

How to Choose the Right Statistical Test

Repeated Measures ANOVA and How It Differs From Ordinary ANOVA

Learn to Perform Repeated Measures One-way ANOVA in Prism

How to Perform a Two Sample T Test

How to Perform Non-Linear Regression in Prism

How to Obtain Descriptive Statistics

How to Generate a Frequency Distribution of Your Data

When to Use a Nonparametric Analysis

How to Choose Between a One-Sided and Two-Sided Test

Learn to Perform Correlation Analysis in Prism

When Do You Use Regression Instead of ANOVA?

Using Data Exploration to Check Statistical Assumptions

Descriptive Statistics in Prism

Learn to Perform Multiple Linear Regression in Prism

When to Use the Grouped Data Table

When to Use the Nested Data Table

Learn to Perform Descriptive Statistics in Prism

Learn to Perform Repeated Measures Two-way ANOVA in Prism

How to Perform Linear Regression in Prism

Learn to do a Chi-square or Fisher’s Exact Test in Prism

When to Use Principal Component Analysis (PCA) in Prism

Fitting a Principal Component Analysis (PCA) Model

Principal Components Regression (PCR)

How to Choose between Regression and Correlation

When Do You Use Regression Instead of ANOVA?

How to Perform a One-way ANOVA in Prism

How to Choose Between A Paired and Unpaired T Test

How to Perform Multiple T Tests in Prism

How to Test for Normality and Lognormality

Chi-Square and Fisher's Exact Tests

How to Fit a Regression Line of Subset Data on a Graph in Prism

Getting Started on the Right Foot with Statistics

Calculating Sample Size With Power Analysis

Understanding Chi-Square and Fisher's Exact Tests in Prism

### Key Concepts of Prism Data Tables

INSIDE THE VIDEO

Prism has eight data tables, each one aligned with a specific set of analyses and graphs. This video will show you how to choose the right table and explain how to enter your data into that table.

LENGTH

5 Minutes

### When to Use the XY Data Table

INSIDE THE VIDEO

Important analyses such as dose-response, nonlinear regression, simple logistic regression, and simple linear regression can only be run from an XY table. Learn more here.

LENGTH

4 Minutes

### When to Use the Column Data Table

INSIDE THE VIDEO

If you have a single categorical variable with two or more groups and a numeric response, you need a Column data table. Watch this video to learn more.

LENGTH

4 Minutes

### When to Use the Grouped Data Table

INSIDE THE VIDEO

If you have two categorical variables and a numeric response, you need a Grouped data table. Important analyses such as two-way and three-way ANOVA and multiple t tests can only be run from a Grouped table. Watch this video for more.

LENGTH

4 Minutes

### When to Use the Multiple Variables Data Table

INSIDE THE VIDEO

If you have more than two numeric columns that you want to relate to each other, you need a Multiple Variables data table. I

LENGTH

4 Minutes

### When to Use the Nested Data Table

INSIDE THE VIDEO

If you have two categorical variables and one variable is nested within the other, then you need a Nested data table. Important analyses such as nested one-way ANOVA and nested t tests can only be run from a nested table.

LENGTH

4 Minutes

### When to Use Contingency, Survival, or Parts of a Whole Data Tables

INSIDE THE VIDEO

Need specific tables for different data types: Contingency for counts, Survival for time-to-event, Parts of a Whole for grouped counts. Vital for tests like Chi-square, Fisher’s exact, and Kaplan-Meier analysis. Learn more in this video.

LENGTH

4 Minutes

### Learn to Perform Descriptive Statistics in Prism

INSIDE THE VIDEO

Learn how to find out more about your data in Prism, including quartiles, median, SD, SEM, confidence interval, coefficient of variation, geometric mean, and more. In addition, this video will cover how to test for normality in your experimental data.

LENGTH

14 Minutes

### How to Perform a T Test in Prism

INSIDE THE VIDEO

Learn the steps required to undertake a t test in Prism. The video also features tips to help you more efficiently navigate and use Prism.

LENGTH

10 Minutes

### Understanding One-Way ANOVA

INSIDE THE VIDEO

It’s easy to produce a p value, but it’s difficult to do good stats. This video walks you through what you should consider before running a one-way ANOVA.

LENGTH

11 Minutes

### Learn to Perform Repeated Measures One-way ANOVA in Prism

INSIDE THE VIDEO

Learn the steps required to perform a one-way repeated measures ANOVA, including how to correctly enter your data and specify where the repeated measures occur in your data.

LENGTH

11 Minutes

### Learn to Perform Repeated Measures Two-way ANOVA in Prism

INSIDE THE VIDEO

Learn when you should use a two-way ANOVA test, as well as the steps to performing the analysis.

LENGTH

13 Minutes

### Learn to Perform a Three-way ANOVA in Prism

INSIDE THE VIDEO

Learn the steps required to perform a three-way ANOVA, including the analysis choices and options available in Prism to help you extract the most information out of your three-factor experiments.

LENGTH

10 Minutes

### How to Perform Linear Regression in Prism

INSIDE THE VIDEO

Learn the steps required to perform a linear regression, including the analysis choices and options available in Prism to help you extract the most information out of your regression analyses.

LENGTH

9 Minutes

### How to Perform a Kaplan-Meier Survival Analysis in Prism

INSIDE THE VIDEO

Learn how to correctly enter failure/censor times and perform a Kaplan-Meier survival analysis in Prism.

LENGTH

12 Minutes

### Learn to do a Chi-square or Fisher’s Exact Test in Prism

INSIDE THE VIDEO

Learn the steps required to perform Chi-square and Fisher’s exact tests in Prism, including how to select and interpret an odds ratio.

LENGTH

8 Minutes

### Principal Component Analysis (PCA) in Prism

INSIDE THE VIDEO

This video introduces Principal Component Analysis, or PCA, a powerful tool used for exploratory analyses with large datasets.

LENGTH

6 Minutes

### When to Use Principal Component Analysis (PCA) in Prism

INSIDE THE VIDEO

Principal Component Analysis is a powerful tool for data exploration and dimension reduction. If you have a dataset that you’re attempting to analyze, this video will help you decide if PCA is an appropriate tool.

LENGTH

6 Minutes

### How Principal Component Analysis (PCA) Works

INSIDE THE VIDEO

For many scientists, PCA is a black box technique. In this lesson, we delve into the primary method underneath PCA, which is the Singular Value Decomposition.

LENGTH

5 Minutes

### Fitting a Principal Component Analysis (PCA) Model

INSIDE THE VIDEO

This lesson walks you through the choices that you’ll need to make to fit a PCA model in Prism.

### Understanding Your Principal Component Analysis Results

INSIDE THE VIDEO

There are a lot of output options for PCA, and in this lesson, we walk you through how to interpret the output. This includes tables of outputted information as well as useful graphics.

LENGTH

5 Minutes

### Principal Components Regression (PCR)

INSIDE THE VIDEO

If your data has too many columns and not enough rows to fit linear regression, PCR may be a good solution. It first fits PCA and then fits PCR on the principal components. Watch this video to learn more.

LENGTH

6 Minutes

### How to Avoid P-Hacking

INSIDE THE VIDEO

Watch this video to learn more about P-Hacking with tips on how you can avoid it.

LENGTH

4 minutes

### How to Choose between Regression and Correlation

INSIDE THE VIDEO

Watch this video to learn about the similarities and differences between correlation and regression and the advantages of each.

LENGTH

4 Minutes

### How to Choose the Right Statistical Test

INSIDE THE VIDEO

This video provides an informative overview of how to choose the right statistical test in Prism for your analyses. Think of this as a decision-making framework for all your data analysis projects in Prism.

LENGTH

4 Minutes

### Dose Response Case Study

INSIDE THE VIDEO

Dose-response experiments in the real-world are rarely straightforward. Learn about each of the parameters in the 4-parameter logistic model and how to identify and solve issues related to ambiguous models, unstable parameters, extrapolation, and outliers.

LENGTH

8 Minutes

### Repeated Measures ANOVA and How It Differs From Ordinary ANOVA

INSIDE THE VIDEO

Learn about some of the ways that repeated measures can appear in an experiment

LENGTH

3 minutes

### When Do You Use Regression Instead of ANOVA?

INSIDE THE VIDEO

Watch this video to learn more about when to use regression instead of ANOVA

LENGTH

2 minutes

### Understanding One-Way ANOVA

INSIDE THE VIDEO

It’s easy to produce a p value, but it’s difficult to do good stats. This video walks you through what you should consider before running a one-way ANOVA.

LENGTH

11 Minutes

### How to Perform a One-way ANOVA in Prism

INSIDE THE VIDEO

Learn the steps required to perform a one-way ANOVA, including what analysis choices and options you will have to make about your experiment to perform the analysis.

LENGTH

11 minutes

### Learn to Perform Repeated Measures One-way ANOVA in Prism

INSIDE THE VIDEO

Learn the steps required to perform a one-way repeated measures ANOVA, including how to correctly enter your data and specify where the repeated measures occur in your data.

LENGTH

11 Minutes

### How to Choose Between A Paired and Unpaired T Test

INSIDE THE VIDEO

Learn to determine whether a paired, unpaired, or one-sample t-test is appropriate in your particular situation

LENGTH

2 minutes

### How to Perform a Two Sample T Test

INSIDE THE VIDEO

Learn how you can use Prism's two sample t test to help check the analysis of your assumptions

LENGTH

10 minutes

### How to Perform Multiple T Tests in Prism

INSIDE THE VIDEO

Did you know that you can perform multiple t tests in Prism, including nonparametric analyses and multiple paired t tests?

LENGTH

5 Minutes

### How to Perform Non-Linear Regression in Prism

INSIDE THE VIDEO

Prism’s nonlinear regression is an incredibly powerful and useful tool with many analysis options. Learn how to perform a nonlinear regression analysis in Prism and see how it can be used to analyze dose-response data.

LENGTH

14 Minutes

### Dose Response Case Study

INSIDE THE VIDEO

Dose-response experiments in the real-world are rarely straightforward. Learn about each of the parameters in the 4-parameter logistic model and how to identify and solve issues related to ambiguous models, unstable parameters, extrapolation, and outliers.

LENGTH

8 Minutes

### How to Transform Data

INSIDE THE VIDEO

Transformations are often required to meet the assumptions for analysis or to improve the appearance and interpretability of a graph. This video shows you how to easily transform your data using Prism’s built-in functions and much more!

LENGTH

15 Minutes

### How to Normalize Data

INSIDE THE VIDEO

Learn the significance of normalization—aligning response scales & setting shared min-max values (e.g., 0-100) across datasets. This video will guide you to normalize efficiently using Prism, covering reasons and rapid steps for normalization.

LENGTH

8 Minutes

### How to Remove Baseline Values

INSIDE THE VIDEO

Discover the process of eliminating baseline values and executing column operations and gain insights into optimal timing for baseline removal, techniques to define values, and the impact on repeated measures and replicates.

LENGTH

10 Minutes

### How to Transpose Data

INSIDE THE VIDEO

Watch this video to learn the steps you need to follow in order to transpose data and review the results in Prism.

LENGTH

8 Minutes

### How to Obtain Descriptive Statistics

INSIDE THE VIDEO

Learn how to summarize your data in Prism, including quartiles, median, SD, SEM, confidence interval, coefficient of variation, geometric mean, and more. In addition, this video will cover how to test for normality in your experimental data.

LENGTH

14 Minutes

### How to Test for Normality and Lognormality

INSIDE THE VIDEO

Learn how to test your data for normality and lognormality in Prism. This video walks you through how to statistically analyze whether or not a data set was drawn from a normal population.

LENGTH

20 Minutes

### How to Generate a Frequency Distribution of Your Data

INSIDE THE VIDEO

Watch this video to learn the steps required to generate a frequency distribution of a data set in Prism.

LENGTH

13 Minutes

### How to Identify Outliers

INSIDE THE VIDEO

This video will guide you through the steps required to detect and remove outliers from data in Prism.

LENGTH

3 Minutes

### When to Use a Nonparametric Analysis

INSIDE THE VIDEO

Learn the benefits and drawbacks of a nonparametric analysis and when it’s important to use one.

LENGTH

2 minutes

### Chi-Square and Fisher's Exact Tests

INSIDE THE VIDEO

Determine if there is a significant difference between two proportions

LENGTH

2 Minutes

### How to Choose Between a One-Sided and Two-Sided Test

INSIDE THE VIDEO

Learn about the differences between one-tailed and two-tailed hypothesis tests and their advantages and disadvantages

LENGTH

2 minutes

### Regression Best Practices

INSIDE THE VIDEO

Regression is a powerful tool, but care needs to be taken when performing the analysis, making predictions, and interpreting the results. In this video, learn when it’s appropriate to use regression and how to avoid common pitfalls.

LENGTH

4 Minutes

### Learn to Perform Correlation Analysis in Prism

INSIDE THE VIDEO

Correlation is used to assess how strong the linear relationship is between two numeric variables. Learn how to perform a correlation analysis in Prism and how to correctly interpret the results.

LENGTH

10 Minutes

### How to Fit a Regression Line of Subset Data on a Graph in Prism

INSIDE THE VIDEO

This video will walk you through how to fit a regression line to a subset of data while still graphing all of the data on a graph.

LENGTH

10 Minutes

### When Do You Use Regression Instead of ANOVA?

INSIDE THE VIDEO

Watch this video to learn more about when to use regression instead of ANOVA

LENGTH

2 minutes

### Getting Started on the Right Foot with Statistics

INSIDE THE VIDEO

Prism is a powerful tool, but without an understanding of the fundamentals of statistics, it can be intimidating. This video gives you the statistical background you need to start performing analyses in Prism with confidence.

LENGTH

5 Minutes

### Using Data Exploration to Check Statistical Assumptions

INSIDE THE VIDEO

Parametric tests, such as the unpaired t test, have important assumptions that need to be verified before an analysis can be carried out. This video discusses those assumptions and how to verify them using statistical tests and data exploration graphs.

LENGTH

10 Minutes

### Calculating Sample Size With Power Analysis

INSIDE THE VIDEO

Sample size plays a critical role in every statistical analysis. Learn how power analysis can help you get a large enough sample size to detect the effect you are looking for while avoiding unnecessarily large sample sizes that waste resources.

LENGTH

11 Minutes

### Descriptive Statistics in Prism

INSIDE THE VIDEO

Summarizing your data into a few useful statistics allows you to quickly and concisely see differences between groups and/or trends over time. Measures of center and spread are covered as well as an introduction to confidence intervals.

LENGTH

9 Minutes

### Understanding Chi-Square and Fisher's Exact Tests in Prism

INSIDE THE VIDEO

Running a Chi-square or Fisher’s exact test will help you determine whether or not there is a significant difference between two proportions. However, there are a few questions you should consider answering before running each test.

LENGTH

2 Minutes

### Learn to Perform Multiple Linear Regression in Prism

INSIDE THE VIDEO

Learn how to perform regression analysis when you have two groups, say treatment and control, and how to test for differences between the two regression models.

LENGTH

10 Minutes

### Regression with Prism Goodness-of-Fit

INSIDE THE VIDEO

Regression is one of the most popular tools in statistics, but how do you know if your regression model is good? Learn how to assess the regression fit using 5 basic techniques.

LENGTH

11 Minutes

### Understanding the Different Types of Data

INSIDE THE VIDEO

Knowing the scale of measurement for your data is an important aspect in choosing the right statistical tool and can help prevent mistakes. In this video, you will learn about the four types of data scales and see examples of each.

LENGTH

4 Minutes

### Understanding Your P Value

INSIDE THE VIDEO

If you are conducting a scientific experiment, the chances are high that you will encounter a p value or two. Learn how to correctly state your conclusions, whether your p value is below or above your cut-off.

LENGTH

2 Minutes

### Repeated Measures Case Study

INSIDE THE VIDEO

The experimental design you choose can have an enormous impact on your statistical results and research findings. You will learn the benefits and drawbacks of five experimental designs for comparing two treatments

LENGTH

6 Minutes

Analyze, graph and present your scientific work easily with GraphPad Prism. No coding required.