# Manuals and step-by-step examples for Prism 5

Note that Prism 5 is several years old. If you use Prism 5, make sure you are up to date.

**Manuals**

Download the Prism 5 Statistics Help Guide (2.3MB pdf)

Download the Prism 5 Regression Guide (2.9MB pdf)

Access the Prism 5 Manual online

**Getting Started**

**Step-by-Step Examples - Nonlinear Regression**

Fitting an enzyme kinetics curve

Comparing two enzyme kinetics models

Interpolating from a sigmoidal standard curve

Automatic outlier elimination (exponential decay)

Global nonlinear regression (two dose-response curves)

Dependency and ambiguous fits (dose-response curves)

**Detailed help to fit built-in equations**

Prism comes with dozens of built-in equations to use in nonlinear regression (of course you can also add your own equations). For each of the built-in equations, we provide a detailed description with step-by-step instructions. Here are a few examples:

Equation: log(agonist) vs. response -- Variable slope

Equation: log(inhibitor) vs. normalized response

Equation: One phase exponential decay

Equation: Competitive binding - One site - Fit Ki

Equation: Receptor binding - Dissociation kinetics

Equation: Mixed-model enzyme inhibition

Equation: Gaddum/Schild EC50 shift

**Step-by-Step Statistical Examples**

Descriptive Statistics

Compare two groups

Unpaired t test from averaged data

Compare three or more groups

Repeated measures one-way ANOVA

Two-way ANOVA

Two-way ANOVA. Repeated measures by row

Two-way ANOVA. Repeated measures by column

Categorical outcomes

Diagnostic lab tests