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Prism 10.5.0

Prism 10.5.0 was released on May 29, 2025.

Discover significant enhancements to simplify survival analysis, improve data interpretation and make collaboration easier than ever.

Automatic Number at Risk Table

Kaplan-Meier plots now include auto-generated Number at Risk table** for easier interpretation

Gain Deeper Insights: Easily determine the number of subjects at risk or censored
Dynamic Updates: Automatically updates to match time or interval changes
Visual Consistency: Colors automatically match your survival curves

**Note this feature is available for GraphPad Prism for Enterprise

Assign editor rights in Prism Cloud

Stay in sync on file versions to improve collaboration

Multi-User Updates: Assign others as editors to publish changes to shared projects
Live Version Awareness: Get alerts if working on outdated files
No More Email Chains: Your work all in one place
Learn more about Prism Cloud

Gain Deeper Insights into Survival Data

Enhance your survival analysis with additional statistical tools for deeper insights

Multiple Pairwise Comparisons*: Simply specify which comparisons Prism should perform and the desired correction methods to receive all necessary statistics, P values and summaries that you need
Confidence Intervals: Obtain the 95% confidence interval for the median survival time for each group

Handle Lognormal Data with Ease

Lognormal data analysis is now easier with built-in tests - no manual transformations required

Why Lognormal Matters

Common in science: lognormal distributions arise naturally when biological effects are multiplicative rather than additive, and are common in pharmacology and many other fields
More statistical power: properly analyzing data from lognormal distributions increases statistical power, requires smaller sample sizes, and prevents false outlier identification
More informative results: report experimental effects intuitively as ratios rather than differences (e.g. "the treatment doubled the activity")

New Lognormal Analysis Options

Lognormal t-tests
(Unpaired, Lognormal Welch’s t test, Lognormal one-sample t test)
Lognormal one-way ANOVA
(Ordinary, Brown-Forsythe and Welch, Repeated Measures)
Results as ratios of Geometric Means... not differences
Prism analysis results now include geometric means, ratios, and appropriate confidence intervals

Note: The ratio paired t test was available in previous versions of Prism

Complete Release Notes

New Features

Introducing the Number at Risk Table for graphs of Kaplan-Meier survival analysis. This feature automatically adds a table displaying the number of subjects at risk (and the cumulative number of subjects censored) for each group at each time point defined by the major ticks of the X axis. If the data or the graph change, the table automatically updates.**
Multiple (pairwise) comparisons for Kaplan-Meier survival analysis*
Prism now reports the 95% confidence interval for the reported median survival time from Kaplan-Meier survival analysis
New experimental design options to support lognormal distribution assumptions for t tests, one-sample t tests, and one-way ANOVA
New axis controls in the Graph Inspector for multiple variables graphs. Quickly select or browse through different variable assignments with real-time graph updates.*
[Prism Cloud] Enabled support for collaborative updates: providing editor permissions to a colleague on a Prism Cloud project will allow them to publish updates to the same project.***
Improved the ability to define the number of decimal places displayed on graph axis labels. The maximum number of decimals available has been increased.*

Feature Improvements

Math-related improvements:

Improvements to survival analysis:
- Added "# deaths/events" and "# censored subjects" tabs to Kaplan-Meier survival analysis results to allow for easy calculation of survival proportions
- Allowed pairwise multiple comparisons after the main log-rank curve comparisons test of the Survival analysis.
- Implemented a 95% confidence interval for the median survival time in Kaplan-Meier survival analysis, enhancing the 'Data summary' results of survival analysis results.
- Enabled reporting of SE of odds/risk ratio in Cox regression analyses
Implemented a baseline correction analysis feature that allows selecting specific datasets for baseline calculations while retaining visibility of all data columns in the results.
Implemented functions for calculating values from non-central t and F distributions
Improved accuracy of the Anderson-Darling normality test p-value calculation
Enabled reporting of SE of odds/risk ratio in Multiple logistic regression
Made Prism report results of parameter significance test in Odds/risk ratio section of Multiple logistic/Cox regression analyses
Renamed the “Heatmap of raw data” graph into “Heatmap of data” graph in the 'Graphs' tab in the 'Parameters: Hierarchical clustering' dialog.
Added the 'Table Analyzed' section to the Curve comparison tab of the Survival analysis results sheet
Added "Distribution assumption" in tabular results of lognormal ANOVA analysis results along with multiple wording improvements
Changed zero or negative hypothetical value to 1.0 in results if the user selects a one-sample ratio test
Changed "ratios" to "ratio" and "B-A" to "B/A" on the Multiple comparison results page for lognormal one-way ANOVA
Replaced "Fisher's LSD test" with "Individual log-rank tests" on the Multiple Comparisons tab of the Survival parameters dialog

Non-math improvements:

Allowed Prism users to create a heatmap of distance matrix with a dendrogram
Allowed users to change the font formatting of labels enabled for symbols on bubble graphs in the Format Graph dialog and the Graph Inspector
Improved the default error bars to be geoMean with geoSD or geoCI when switching from a linear to a logarithmic scale in XY, Grouped, and Column graphs.
Enhanced dendrogram creation algorithm that sorts subtrees based on average distance, ensuring visually appealing and interpretable results
Implemented an activity indicator when the response from the Prism Cloud server takes more than 2 seconds
Improvements in how Prism handles error use cases when the disk is out of space. Improved the alert that appears on an attempt to save a project while the system disk is out of space.
Improved the Prism auto-recovery functionality to be able to open Prism legacy files with corrupted sheet IDs.
Updated Prism behavior to display all error bars regardless of summary symbol size
(Windows) Enabled formatting of data points on the 'Box & Whiskers' graphs by enabling corresponding commands for the source data table.
(Windows) Made 'Censored points only' the default option for plotting symbols in the 'Change Graph Type' dialog for survival graphs instead of 'All points'
(Mac) Added the 'Writing Tools' sub-menu with Apple Intelligence Beta feature to the contextual menu for text objects within the application

Performance

Improved the performance (compared to previous Prism 10 versions) of copying a graph that plots data from several result sheets if the source tables contain empty data sets.
(Windows) Improved the performance of Fisher's Exact test calculation for large total value by a few times compared to Prism 10.4 and older.
(Windows) Improved the performance of deleting the group of sheets with the ‘Show combined Data+Results folder' view mode enabled for the Navigator panel so that it is essentially faster than Prism 9.
(Windows) Improved the performance of opening Prism documents while the ‘Show combined Data+Results folder' view is enabled in the Navigator panel.

Analysis-related Bug Fixes

Fixed the issue when the Geisser-Greenhouse correction did not apply to the interaction term in the RM Two-way ANOVA with one factor.
Fixed the issue when the P value was incorrectly reported as 000e+000 in ANOVA post-tests.
Fixed the issue when 'Mean diff. CI plot' results tab and graph became empty if the corresponding option was unchecked and then selected again in the 'Parameters: Nested t test' dialog
Fixed the issue when the 'ANOVA results' tab was absent in the results sheet of the 'K-means clustering' analysis.
Fixed the issue when the 'Nested t test' section of the 'Tabular results' tab contained incorrect results if the 'Fit the full model anyway' option was selected in the 'Parameters: Nested t test' dialog and variance of random effect was negative.
Fixed the issue when the 'Table Analyzed' section was not updated in the result sheet of 't tests' analysis after inserting an empty data set to the data table.
Fixed the issue where the Heatmap tab of the Unpaired t test analysis results appeared empty if the source data table had subcolumns
Fixed the "Family-wise alpha threshold" controls in the Multiple Comparisons tab by excluding confidence interval controls
Fixed the issue when the results tab with an empty title appeared in the 'Nested t test' results sheet in a re-opened project if data was removed from the data table
(Mac) Fixed the issue when 'Within each column, compare rows (simple effects within columns)' kind of comparison was enabled in the 'Parameters: Two-Way ANOVA (or Mixed Model)' dialog for RM model without the interaction term.
(Mac) Fixed the issue when incorrect data sets were used in the 'Remove Baseline and Column Math' analysis if some of the data sets in the input table were not selected for analysis

Graphing Bug Fixes

Fixed an issue where custom settings for data point colors and shapes were incorrectly applied to subsequent datasets after excluding values from the source data table in Grouped - Interleaved/Separated scatter graphs.
Fixed an issue where labels in the Dendrogram graph became misaligned when the aspect ratio was stretched horizontally, ensuring they now correctly position below the branch starts.
Fixed an issue where the fill color legend for bubble plots with a double gradient colormap would change unexpectedly after saving a project.
Fixed the issue when a small value in scientific format instead of 0 was displayed for axes numbering on multiple variables graphs.
Fixed the issue when adding Compact Letter Display labels on the graph from the 'Two-way ANOVA' results sheet was impossible if one of the compared samples was empty.
Fixed the issue when curves generated by analyses did not reappear on the graph after deleting and re-entering data in the source data table.
Fixed the issue when spikes plotted for negative values disappeared from the XY graph if they did not fit the Y-axis range.
Fixed the issue when symbols from the data table and curves generated by Nonlinear and Simple Linear regression analyses had different colors on the XY graph if an empty data set was added to the source data table.
Fixed the issue where the magic wand feature did not carry over formatting from one graph to another when data symbols on the graph were turned off.
Fixed the issue when the 'Reverse Color Legend' command in the contextual menu on the multiple variable heatmap graph did not reverse the color legend.
Fixed the issue when the color legend of the categorical variable was not removed from the graph if the corresponding variable type was changed to continuous in the data table.
Fixed the issue when it was impossible to change the style of dendrogram branches to a solid line after applying any style for all clusters at once in the 'Format Graph' dialog or the Inspector.
Fixed the issue when the dendrogram orientation unexpectedly rotated on the multiple variables heatmap graph after undoing the assignment of variables to the X and Y axes.
Fixed the issue when the legends and labels font on the Dendrogram graph did not match the font set in 'Preferences' after performing Hierarchical clustering analysis.
Fixed the issue when the X-axis appeared on the Dendrogram graph after changing the thickness of the distance axis using the contextual menu.
Disabled the 'Fill Pattern' options for embedded tables on multiple variables graphs.
Fixed the issue when logarithmic minor ticks were not displayed correctly on multiple variables graphs if the 'Log 2' scale was applied to the graph axes.
Fixed the issue when the graph appeared blank without curves generated by the Nonlinear regression analysis with equations that define X=0 and the default range if all X values were negative
Fixed the issue in which additional Dendrogram and Heatmap graphs unexpectedly appeared for a Hierarchical clustering analysis after recalculating results in a re-opened project created in a previous version of Prism
Fixed the issue when no data appeared on the multiple variables plot after assigning other data sets to the X/Y axis if the previous ones were deleted from the data table and the project was saved with an empty graph.
(Windows) Fixed the issue when bar fill colors were displayed as semi-transparent instead of solid on graphs with a user-defined color scheme applied.
(Windows) Fixed the issue when colors of a graph's elements were unexpectedly reset in the ‘Data Sets’ tab of the ‘Define Color Scheme’ dialog after changing color for the ‘Bar pattern.’
(Windows) Fixed the issue when not all minor ticks were shown on the axis with a custom range if the last major tick was out of that range.
(Windows) Fixed the issue when symbols in the Interleaved bars graph did not change after formatting data points from the results sheet if the data table contained empty cells.
(Windows) Fixed an issue where changing the fill color of bars in a Scatter with bar graph did not apply the selected color.
(Windows) Fixed the issue when the 'Reverse Color Legend' command in the contextual menu reversed the order of the Size legend on bubble plots.
(Windows) Fixed the issue when data sets from the source data table were removed from the graph sheet after deleting one of the linked results sheets.
(Windows) Fixed the issue when changes applied for embedded tables were not displayed in the preview of the "Magic Step 2" dialog
(Windows) Fixed the issue when error bars did not appear on the survival graphs after applying the "SE" or "95% CI" option in the "Error bars" section of the "Format Graph" dialog
(Windows) Fixed the issue when formatting was not applied to the embedded table on the target graph after performing Magic
(Windows) Fixed the issue with the alignment of the vertical arrow in the "Timeline" graph Portfolio file
(Windows) Fixed the issue when the 'Show Color Legend' item was grayed out in the contextual menu opened on the multiple variables heatmap and dendrogram graphs after it was hidden.
(Windows) Fixed the issue when annotations were not displayed on Scatter and Before-after column graphs.
(Windows) Made it possible to format data points on the 'Box and Whiskers' graph from the source data table.
(Mac) Fixed an issue where the colors and parameters of symbols on bubble plots with the double gradient colormap changed unexpectedly after saving the Prism projects.
(Mac) Fixed an issue where minor ticks were incorrectly displayed on the X axis of grouped interleaved bar graphs when the axis format was set to 'X values' and the major ticks interval was altered.
(Mac) Fixed an issue where double-clicking on the X/Y axis in the classic heat map invoked the 'Format Axes' dialog instead of the 'Format Graph' dialog.
(Mac) Fixed the issue when the Y axis title unexpectedly moved closer to the axis in reopened projects saved in .pzfx/.pzf formats if Y axis had an additional tick with a long title.
(Mac) Fixed an issue when the fill pattern of bars in exported graphs appeared wider than in the original Prism graph when using HiDPI display resolutions.

Other Bug Fixes

Fixed the issue in which Prism would crash when switching to the results sheet after adding new categories to a categorical variable in the data.csv file of a .prism project.
Fixed the issue where graphs were not generated by an analysis when a project contained 500 graph sheets, instead ensuring that an appropriate alert regarding the graph sheet limit is shown before performing the analysis.
Fixed the issue when no constant was hooked to the graph sheet from the 'Identify outliers' analysis with the 'Perform the calculation for each subcolumn separately' option selected.
Fixed the issue when the floating note contained an incorrect maximal number of data sets in the data table for the 'Dose-response curves' sample from the Graph Portfolio.
Fixed the issue when the levels from the categorical variable set for the ‘Metric Variable’ of the multiple variable heatmap graph were not updated in the legends and Colormap Group dropdown in the Inspector and the Format Graph dialog.
Fixed the issue where tooltips with coordinates did not appear when dragging a line handle in bubble plot graphs
Fixed the issue when the embedded data table on the bubble plot lost its link to the source data table after grouping with any drawing.
Fixed the issue when additional Dendrogram and Heatmap graphs unexpectedly appeared for Hierarchical clustering analysis after recalculating the results in the re-opened project.
Fixed the issue in which Prism would crash upon creating an analysis using the Wand feature for a data table with linked data
Fixed the "Interpret" toolbar button so that it correctly leads to the corresponding checklist pages of the User Guide when the lognormal distribution was used for an analysis
Fixed the issue when part of the data was lost from the data table after resaving the project if the data hash was invalid and the value of the 'lastRow' parameter was less than the number of rows in the corresponding CSV file
Improved heading formatting in the license notice.txt file
(Windows) Fixed the issue in which Prism would crash on opening legacy (pzfx) files with corrupted sheet IDs.
(Windows) Fixed the issue when applying the 'Wand' tool on multiple data tables in gallery view mode led to the creation of an excessive number of analysis results.
(Windows) Fixed an issue in which Prism's activation was lost while using several instances of the application.
(Windows) Fixed the issue when X values represented as a series were omitted while pasting as linked values to the data table.
(Windows) Fixed the issue where black labels appeared as red labels in exported EPS files from the clustered data graph.
(Windows) Fixed the issue in which Prism would crash on opening projects that contained several multiple variables graphs via script if projects were saved in the gallery view mode in .prism format.
(Windows) Fixed the crash on invoking the 'Analyze' main menu from the 'Narrative results' or 'Are lines different?' results tab of 'Two-way ANOVA' and 'Simple linear regression' analyses.
(Windows) Fixed the crash on applying changes in the 'Format Data Table' dialog if multi-lined subcolumn titles were entered.
(Windows) Fixed the issue when the Notes section in the duplicated Info sheet appeared empty when duplicating a family from the Info sheet.
(Windows) Fixed the crash on performing Export operation from empty Graphs or Layouts sections in the Navigator.
(Windows) Fixed the issue when line spacing and text justification toolbar buttons were disabled for text in floating notes on multiple variables graphs, preventing users from changing text styles and colors.
(Windows) Fixed the issue when the 'Text Line Spacing' preferences option did not affect the text of newly created floating notes.
(Windows) Fixed the crash on deleting a new unsaved script in the 'GraphPad Prism Script' dialog.
(Windows) Fixed the crash on cloning a graph when the data table contained multiple-row text in row titles.
(Windows) Fixed the issue when the alert about unsaved changes appeared on closing the project after switching between sheets without making any changes.
(Windows) Fixed the crash that occurred when clicking the OK button in the Parameters dialog invoked from multiple variables graphs which were generated by "Principal Component Analysis", "K-means clustering", or "Hierarchical clustering" analyses and contained an embedded table
(Windows) Fixed the crash that would occur upon deleting a multiple variables graph with a horizontal fill color legend
(Windows) Fixed the crash that would occur upon invoking the "Parameters: Simple Survival Analysis" dialog from the results sheet created after duplicating the survival graph linked to an orphaned results sheet
(Windows) Fixed the crash that occurred upon performing the Redo operation after undoing several changes related to showing error bars on a survival graph
(Windows) Fixed a typo on the "Credits" tab of the "About GraphPad Prism" dialog
(Windows) Fixed the issue when nothing happened on pasting an embedded table on the graph sheet after it was cut.
(Windows) Fixed the issue when the 'Publish to Prism Cloud' popup/dialog was displayed instead of the 'Previously Published' one on publishing a reopened project to Prism Cloud if it was previously published by another user.
(Windows) Fixed the issue when the default font set for floating notes in Preferences was not applied to the text of newly created floating notes.
(Mac) Fixed the crash on closing .prism project with the pairwise comparison after it was previously saved as a family in the .pzfx format.
(Mac) Fixed the crash on applying the color scheme to the dendrogram from the Graph Inspector if a single color was selected for all branches and the Undo operation was performed for the previously applied color scheme.
(Mac) Fixed the crash on deleting the data table sheet if the related graph sheet contained a bubble plot with Confidence Ellipses or Convex Hulls data objects.
(Mac) [macOS 10.14] Fixed the crash upon opening the Prism document that contained a multiple variables graph.
(Mac) Fixed the issue when pop-ups of Writing Tools with Apple Intelligence Beta were not invoked for text objects on graph and layout sheets.
(Mac) Fixed the issue where controls were cut off in the Size sub-tab of the Data tab in the Inspector for multiple variables graphs.
(Mac) Fixed the issue when the border thickness value of axes labels displayed in the Inspector panel did not correspond to the actual border thickness on the graph.
(Mac) Fixed the rendering issue where the ‘Metric variable’, ‘Thousands’ and ‘Y axis labels’ dropdown menus in the Inspector for the Heatmap graphs lacked bottom shadows.
(Mac) Fixed the issue where the font selection popup in the Inspector unexpectedly shifted down when returning to the Labels tab while on the Dendrogram graph.
(Mac) Fixed the issue where the prefix was not applied to names of sheets created by using a saved method in the 'Analyze Data' dialog.
(Mac) Fixed the issue when the 'Transparency' value unexpectedly changed to 0% after applying the color scheme in the 'Format Graph' dialog for a multiple variables graph.
(Mac) Fixed the issue where the page background color box in the Inspector was not updated after selecting a new color scheme.
(Mac) Fixed the issue when the 'Expected #' title was truncated in the 'Expected distribution' section of the “Parameters: Compare observed distribution with expected“ dialog.
(Mac) Fixed the crash that would occur upon clicking the "Cancel" button in the "Format Axes" dialog invoked for a blank multiple variables heat map graph
(Mac) Updated Prism to show an alert about the impossibility of saving the file if there was not enough disk space on the system disk when closing a modified .prism project with pasted images on a layout sheet (thus preventing them from being removed unexpectedly)

* Requires a named-user Prism license (not available for serial number-based Prism licenses)

** Requires a Prism Enterprise license

*** Requires a Prism subscription with an associated Prism Cloud Workspace

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