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Lineweaver-Burk analysis is one method of linearizing substrate-velocity data so as to determine the kinetic constants Km and Vmax. One creates a secondary, ...

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It is the substrate concentration needed to achieve a half-maximum enzyme velocity. Create a Lineweaver-Burk plot. Before nonlinear regression was available, ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

We don't recommend this method of creating a Lineweaver-Burk plot (see this step-by-step alternative procedure). But if you really want to do this, ...

GraphPad FAQs

Scatchard, Lineweaver-Burk, and similar transformations are fine for visualization, but we discourage linearization of data followed by linear regression for ...

GraphPad FAQs

I followed the manual instructions for making a Lineweaver-Burk plot, but the line isn't right. Why? There was an error in the steps presented in the Help for ...

GraphPad FAQs

Before analyzing your data with linear regression, stop and ask yourself whether it might make more sense to fit your data with nonlinear regression.

https://www.graphpad.com/guides/prism/latest/curve-fitting/

The resulting 95% CI will be asymmetrical. Transforms for pharmacology and biochemistry. Eadie-Hofstee, Hanes-Woolf, and Lineweaver-Burk transforms ...

https://www.graphpad.com/guides/prism/latest/user-guide/

Don't use hinge function to analyze a biphasic Scatchard or Lineweaver-Burk plot. A biphasic Scatchard plot follows a curve, not two intersecting lines ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Don't use segmental linear regression to analyze a biphasic Scatchard or Lineweaver-Burk plot. A biphasic Scatchard plot follows a curve, not two ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

You can easily customize tick labels in Prism (any version). For example, on the Lineweaver-Burk plot (below), you may wish to dispense with X-axis labels ...

GraphPad FAQs