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Computing Kcat by hand. If you plot enzyme velocity as a function of subtrate concentration, you can fit the data to the Michaelis-Menten equation to ...

GraphPad FAQs

... Burk) plots shown in the Prism manual? Why not use linear regression?(127) ... I'm trying to make a Lineweaver-Burk plot using linear regression. How do ...

GraphPad FAQs

https://www.graphpad.com/guides/prism/8/pdf/

Introduction Kcat is the turnover number -- the number of substrate molecule each enzyme site converts to product per unit time.

https://www.graphpad.com/guides/prism/latest/curve-fitting/

On the Fit tab of the nonlinear regression dialog, open the equation folder, Enzyme Kinetics - Substrate vs. Velocity. Then choose the Michaelis-Menten ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

When you first create a layout page, Prism asks you to choose an arrangement of placeholders. But you are not stuck with this arrangement.

https://www.graphpad.com/guides/prism/latest/user-guide/

You've created a graph and instructed Prism to plot error bars. But when you look at the graph, you notice that some of the error bars aren't visible. You may ...

GraphPad FAQs

Introduction If the enzyme has cooperative subunits, the graph of enzyme velocity as a function of substrate concentration will appear sigmoidal.

https://www.graphpad.com/guides/prism/latest/curve-fitting/