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Problem: In your step-by Step Example: Analyzing RIA and ELISA Data using Prism 3. I followed the steps in your instruction booklet. Six standard points are ...

GraphPad FAQs

The EC50 is the concentration of a drug that gives half-maximal response. The IC50 is the concentration of an inhibitor where the response (or binding) is ...

GraphPad FAQs

Prism makes it very easy to interpolate unknown values from a standard curve. Enter the standards with both X and Y values, fit a line or curve.

https://www.graphpad.com/guides/prism/latest/curve-fitting/

The usual steepness of a dose-response curve Many dose-response curves follow the shape of a receptor binding curve. As shown below, 81 times more agonist ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

When developing (or assessing) an assay to test the effectiveness of various drugs, you want to quantify how well the assay works. One way to do this is via ...

GraphPad FAQs

RIA/ELISA analysis · User defined equations · Standard curve · PowerPoint and Prism · Templates. Keywords: tutorials. Explore the Knowledgebase. Analyze, graph ...

GraphPad FAQs

If your dilution factor values take into account all serial dilution steps and can be related to a single stock concentration, then this is easy to do using ...

GraphPad FAQs

It is used as a cumulative measurement of drug effect in pharmacokinetics and as a means to compare peaks in chromatography. Note that Prism also computes the ...

https://www.graphpad.com/guides/prism/latest/statistics/

Reasons for all interpolated values to be blank If the nonlinear regression procedure did not converge on a best-fit curve, then Prism will leave all ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Prism will extrapolate only a limited distance beyond the data. Usually, it will extrapolate in each direction up to half the range of the X axis.

https://www.graphpad.com/guides/prism/latest/curve-fitting/