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It fits four parameters: the bottom and top plateaus of the curve, the EC50 (or IC50), and the slope factor (Hill slope). This curve is symmetrical around ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

The best-fit curve will be identical, but the parameter values will differ. The best fit value of the "EC50" will be very different.

GraphPad FAQs

EC50 is the concentration of agonist that gives a response half way between ... Depending on which units Y is expressed in, and the values of Bottom and Top, the ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

It fits four parameters: the bottom and top plateaus of the curve, the EC50 (or IC50), and the slope factor (Hill slope). This curve is symmetrical around ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

EC50 is the concentration of agonist that gives a response half way between ... Depending on which units Y is expressed in, and the values of Bottom and Top, the ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

EC50 and LogEC50: EC50 is the concentration of agonist that gives half maximal response in the absence of inhibitor. The logEC50 is in the same units you used ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

The problem is that Prism 4 tries to recalculate the EC50 from the LOGEC50. But Prism 5 saved it as "LogEC50" which Prism 4 does not recognize, due to the lower ...

GraphPad FAQs

Equation: EC50 shift; Equation: Allosteric EC50 shift; Equation: ECanything; Equation: Absolute IC50. Dose-response -- Special (X is Log[Concentration]) ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

The graph below shows two dose-response curves. The goal of the experiment is to determine the two EC50 values. The EC50 is the concentration (dose) that ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

The EC50 is determined by two properties of the agonist: •How well it binds to the receptor, quantified by the affinity of the drug for binding to its ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/