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The EC50; Confidence intervals of the EC50; Hill slope; Choosing a dose-response equation; Pros and cons of normalizing the data; The term "logistic"; 50% of ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

So long as you enter a 'concentration' more than three log units below the EC50 (and assuming a Hill Slope not too far from 1.0), the usual method works ...

GraphPad FAQs

•The best-fit value of an EC50 determined by nonlinear regression. •The ratio of the median survival times of two groups. •The median of a set of values. The ...

https://www.graphpad.com/guides/prism/latest/statistics/

... EC50, but you also want to include a value and error estimate for the EC50 based on the best-fit model parameter values. Enter the label “EC50” in the left ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

For this purpose, you don't much care about the meaning of the model, or the interpretation of the best-fit values of the parameters (EC50, etc.). 3.For ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

In this case, the equation fits the EC50, rather than the LogEC50. However, for both sets of equations, Prism will report both the LogEC50 and the transformed ...

GraphPad FAQs

So fitting to a biphasic model -- with two EC50 values -- is ambiguous. Model has redundant parameters. The simplest example would be fitting this model: Y ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

All the ideas can be applied to stimulatory curves and EC50 (E for effective) as well. Just stand on your head when you view the figures!. The ideal ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Equation: EC50 shift; Equation: Allosteric EC50 shift; Equation: ECanything; Equation: Absolute IC50. Dose-response -- Special (X is Log[Concentration]) ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Make sure you pick the form of the equation to match the data you are analyzing. The uncertainty in the value of the EC50 or IC50 is usually quite asymmetrical.

https://www.graphpad.com/guides/prism/latest/curve-fitting/