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Equation: Absolute IC50. Receptor binding - Key concepts; Receptor binding - Saturation binding; Receptor binding - Competitive binding; Receptor binding ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Prism reports that the upper confidence limit is infinity when the model and data simply don't define the upper confidence limit.

GraphPad FAQs

Equation: Absolute IC50. Receptor binding - Key concepts; Receptor binding - Saturation binding; Receptor binding - Competitive binding; Receptor binding ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

(This midpoint is sometimes called the IC50 or ED50). First let's look at the variables without a log transform, H+ and EC50. Both come pretty close to the ...

GraphPad FAQs

Equation: Absolute IC50. Dose-response -- Special (X is Log[Concentration]); Receptor binding - Key concepts; Receptor binding - Saturation binding; Receptor ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

New: Absolute IC50 equation added to Nonlinear Regression Equation library. The absolute IC50 is the concentration that gives a response of 50%, which is ...

https://www.graphpad.com/updates/

Enter a low value (say -10 on the log scale), and then use custom ticks to label the graph correctly (so it is labeled "0" rather than "-10".

GraphPad FAQs

Curve fitting without a model The term curve fitting is more general than regression. Your approach to curve fitting depends on your goal.

https://www.graphpad.com/guides/prism/latest/curve-fitting/

What does "not converged" mean? Nonlinear regression works iteratively. Prism starts with initial estimated values for each parameter.

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Do not ignore the confidence intervals In most cases, the entire point of nonlinear regression is to determine the best-fit values of the parameters in the ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/