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A confidence interval consists of two confidence limits. In some cases, Prism can only determine one confidence limit and reports the other limit as "???".

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Equation: Absolute IC50. Receptor binding - Key concepts; Receptor binding - Saturation binding; Receptor binding - Competitive binding; Receptor binding ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

... IC50 here). For this, the interpolation definition would be: •Absolute IC50 = X[50]. Advanced example: You want to calculate the value of Y that is halfway ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Enter a low value (say -10 on the log scale), and then use custom ticks to label the graph correctly (so it is labeled "0" rather than "-10".

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Equation: Absolute IC50. Receptor binding - Key concepts; Receptor binding - Saturation binding; Receptor binding - Competitive binding; Receptor binding ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Heat maps are a standard way to plot grouped data. The basic idea of a heat map is that the graph is divided into rectangles or squares, each representing ...

https://www.graphpad.com/guides/prism/latest/user-guide/

Equation: Absolute IC50. Dose-response -- Special (X is Log[Concentration]); Receptor binding - Key concepts; Receptor binding - Saturation binding; Receptor ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Prism provides two approaches for fitting a curve without selecting a model. From a table or graph of XY data, click Analyze, and then choose 'Fit spline/ ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Equation: Absolute IC50. Dose-response -- Special (X is Log[Concentration]); Receptor binding - Key concepts; Receptor binding - Saturation binding; Receptor ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Outlier elimination is misleading when you are fitting the wrong model. The left panel above shows the data fit to a dose response curve.

https://www.graphpad.com/guides/prism/latest/curve-fitting/