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This example shows the basics of creating and customizing an XY graph (for this example, a set of two dose-response curves).

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Instead you enter the concentration of radioligand and its Kd as constants, and Prism directly fits the Ki of your cold compound. If you want to know the IC50, ...

https://www.graphpad.com/guides/prism/7/curve-fitting/

Relative vs absolute IC50; Fitting the absolute IC50; Incomplete dose-respone curves; Troubleshooting fits of dose-response curves. Dose-response - Stimulation ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

If you want to fit the two IC50 values instead of the Ki values, use a different equation. The analysis assumes that you know the affinity of both sites for ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

(This midpoint is sometimes called the IC50 or ED50). First let's look at the variables without a log transform, H+ and EC50. Both come pretty close to the ...

GraphPad FAQs

An alternative to the concept of "ambiguous" fits Starting with Prism 8.2, we offer a number of choices regarding how Prism should handle cases when the ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Choose an equation where X is expected to be dose or concentration, and Prism will fit the EC50 or IC50 directly, with standard errors.

GraphPad FAQs

Equation: Absolute IC50. Receptor binding - Key concepts; Receptor binding - Saturation binding; Receptor binding - Competitive binding; Receptor binding ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Equation: Absolute IC50. Receptor binding - Key concepts; Receptor binding - Saturation binding; Receptor binding - Competitive binding; Receptor binding ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Equation: Absolute IC50. Receptor binding - Key concepts; Receptor binding - Saturation binding; Receptor binding - Competitive binding; Receptor binding ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/