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If your dilution factor values take into account all serial dilution steps and can be related to a single stock concentration, then this is easy to do using ...

GraphPad FAQs

The EC50 is the dose of a drug that provokes a response half-way between the top plateau and bottom plateau of the dose response curve.

GraphPad FAQs

Many log(dose) response curves follow the familiar symmetrical sigmoidal shape. The usual goal is to determine the EC50 of the agonist - the concentration that ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

When you ask Prism to interpolate X values from Y values you enter, it will leave the result blank in three cases.

GraphPad FAQs

What are residuals? Unweighted fits A residual is the distance of a point from the curve. Least-squares regression works to minimize the sum of the squares ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Where are the interpolated results? Prism places the interpolation results into its an additional results page (or two additional pages).

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Perform Calculations on the entire row or for each data set column individually.

https://www.graphpad.com/guides/prism/latest/statistics/

The standard curve is sometimes called a four-parameter logistic model, so the asymmetrical curve is called a five parameter logistical model.

GraphPad FAQs

Q&A about R2 What does R2 quantify The value R2 quantifies goodness of fit. It compares the fit of your model to the fit of a horizontal line through the ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

The importance of constraints Prism lets you constrain each parameter to a constant value, constrain to a range of values, share among data sets (global ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/