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You have a choice, in the Confidence tab, to either mark selected parameters as "unstable", to mark entire fits as "ambiguous", or neither.

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Are you fitting the relative IC50 but expecting an absolute IC50, and so are surprised by the results. © 1995-2025 GraphPad Software, LLC. All rights ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

This calculator computes EC 80 or EC 90 (etc.) from EC 50. How? 1. Enter EC 50 I will enter: EC 50 as concentration. For 1nM, enter '1e-9'.

https://www.graphpad.com/quickcalcs/ecanything1/

This is not the same as Vmax,which would require a maximal concentration of substrate. Ki is the inhibition constant, expressed in the same units as X. The IC50 ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

It fits four parameters: the bottom and top plateaus of the curve, the EC50 (or IC50), and the slope factor (Hill slope). This curve is symmetrical around ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

The only difference is that the inhibitory equations fit the IC50 ("I" for inhibition) while the stimulation equations fit the EC50 ("E" for effective). If ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Make sure you pick the form of the equation to match the data you are analyzing. The uncertainty in the value of the EC50 or IC50 is usually quite asymmetrical.

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Additional output (legacy for old Prism versions). The options here (dose ratios, and Ki from IC50) are available only to be compatible with earlier versions of ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

With different kinds of variables, this variable is sometimes called ED50 (effective dose, 50%), or IC50 (inhibitory concentration, 50%, used when the curve ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

It fits four parameters: the bottom and top plateaus of the curve, the EC50 (or IC50), and the slope factor (Hill slope). This curve is symmetrical around ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/