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Prism labels the fit 'ambiguous'. Instead of reporting the confidence interval of some parameters, it simply reports "very wide". The best-fit value of the EC50 ...

GraphPad FAQs

The EC50; Confidence intervals of the EC50; Hill slope; Choosing a dose-response equation; Pros and cons of normalizing the data; The term "logistic"; 50% of ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

The usual equations to fit dose response curves have four parameters (top, bottom, EC50, Hill Slope), and define symmetrical curves.

https://www.graphpad.com/guides/prism/latest/curve-fitting/

The parameter Ka is the EC50, the concentration that provokes a response halfway between Bottom and Top. As written, that equation includes the parameter ...

GraphPad FAQs

The EC50; Confidence intervals of the EC50; Hill slope; Choosing a dose-response equation; Pros and cons of normalizing the data; The term "logistic"; 50% of ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

The EC50; Confidence intervals of the EC50; Hill slope; Choosing a dose-response equation; Pros and cons of normalizing the data; The term "logistic"; 50% of ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Check that EC50 values are within the range of your data. Check that maximum plateaus aren't too much higher than your highest data point. If the best-fit ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

For dose-response curves, you may want to test whether the two EC50 values differ significantly, whether the maximum responses differ, or both. With kinetic ...

GraphPad FAQs

If the definitions of 0% and 100% are ambiguous, then so is the definition of "50%", and thus the EC50 is also ambiguous. Just because you chose to ...

GraphPad FAQs

... EC50), and a parameter that describes how steep the curve is. In Prism, this equation is called the log(agonist) vs response curve -- Variable slope (four ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/