Search Results

I ran 10,000 simulations, and found that in every case the fit worked well and gave a reasonable answer (with the EC50 within the range of the data). No ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Equation: EC50 shift; Equation: Allosteric EC50 shift; Equation: ECanything; Equation: Absolute IC50. Dose-response -- Special (X is Log[Concentration]) ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Make sure you pick the form of the equation to match the data you are analyzing. The uncertainty in the value of the EC50 or IC50 is usually quite asymmetrical.

https://www.graphpad.com/guides/prism/latest/curve-fitting/

The usual equations to fit dose response curves have four parameters (top, bottom, EC50, Hill Slope), and define symmetrical curves.

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Khalf is the concentration of substrate that produces a half-maximal enzyme velocity. It is the EC50. h is the Hill slope. When h=1, this equation is identical ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

The EC50; Confidence intervals of the EC50; Hill slope; Choosing a dose-response equation; Pros and cons of normalizing the data; The term "logistic"; 50% of ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

If the definitions of 0% and 100% are ambiguous, then so is the definition of "50%", and thus the EC50 is also ambiguous. Just because you chose to ...

GraphPad FAQs

For dose-response curves, you may want to test whether the two EC50 values differ significantly, whether the maximum responses differ, or both. With kinetic ...

GraphPad FAQs

... EC50), and a parameter that describes how steep the curve is. In Prism, this equation is called the log(agonist) vs response curve -- Variable slope (four ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

You have a choice, in the Confidence tab, to either mark selected parameters as "unstable", to mark entire fits as "ambiguous", or neither.

https://www.graphpad.com/guides/prism/latest/curve-fitting/