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The EC50; Confidence intervals of the EC50; Hill slope; Choosing a dose-response equation; Pros and cons of normalizing the data; The term "logistic"; 50% of ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

For this purpose, you don't much care about the meaning of the model, or the interpretation of the best-fit values of the parameters (EC50, etc.). 3.For ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Make sure you pick the form of the equation to match the data you are analyzing. The uncertainty in the value of the EC50 or IC50 is usually quite asymmetrical.

https://www.graphpad.com/guides/prism/latest/curve-fitting/

So long as you enter a 'concentration' more than three log units below the EC50 (and assuming a Hill Slope not too far from 1.0), the usual method works ...

GraphPad FAQs

For dose-response curves, you may want to test whether the two EC50 values differ significantly, whether the maximum responses differ, or both. With kinetic ...

GraphPad FAQs

The usual equations to fit dose response curves have four parameters (top, bottom, EC50, Hill Slope), and define symmetrical curves.

https://www.graphpad.com/guides/prism/latest/curve-fitting/

I ran 10,000 simulations, and found that in every case the fit worked well and gave a reasonable answer (with the EC50 within the range of the data). No ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

The EC50; Confidence intervals of the EC50; Hill slope; Choosing a dose-response equation; Pros and cons of normalizing the data; The term "logistic"; 50% of ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Since the uncertainty of the EC50 is very asymmetric, be sure to choose to compute the confidence intervals using the likelihood ratio asymmetric method.

https://www.graphpad.com/guides/prism/latest/curve-fitting/

... EC50), and a parameter that describes how steep the curve is. In Prism, this equation is called the log(agonist) vs response curve -- Variable slope (four ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/