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The results make no sense if the top plateau of a sigmoid curve is far larger than the highest data point, or an EC50 is not within the range of your X values.

https://www.graphpad.com/guides/prism/latest/curve-fitting/

This model fits the Ki of the unlabelled ligand directly. It does not report the EC50, so you do not need to apply the Cheng and Prusoff correction(1).

https://www.graphpad.com/guides/prism/latest/curve-fitting/

So fitting to a biphasic model -- with two EC50 values -- is ambiguous. Model has redundant parameters. The simplest example would be fitting this model: Y ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

If the definitions of 0% and 100% are ambiguous, then so is the definition of "50%", and thus the EC50 is also ambiguous. Just because you chose to ...

GraphPad FAQs

“EC50” is also reported, but it is not a fitted value; Prism simply reports the antilog of the best-fit value for EC50. Users frequently protest the fact ...

GraphPad FAQs

With nonlinear regression of a normalized dose-response curve, the goal is to adjust the values of the EC50 (the concentration that provokes a response ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

We use the term EC50 to be consistent with the equations for the other sigmoid curves. The term IC50 is used more frequently ("E" stands for effective; "I ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

You need to decide when those likelihood are far enough apart that you will believe the parameters are different. Keywords: EC50 IC50 ED50 logIC50 logED50 log ...

GraphPad FAQs

This is a modification of the usual one-site binding equation (rectangular hyperbola), except X and Ka are raised to the nth power. The parameter Ka is the EC50 ...

GraphPad FAQs

Note that the model does not assume that the two EC50 values are the same, but it does assume the two Hill Slopes are the same. Here is a user-defined model ...

GraphPad FAQs