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Prism 3 -- Analyzing RIA and ELISA Data · Enter the Data. In the Welcome to Prism dialog box, select Create a new project and Work independently. · Transform ...

GraphPad FAQs

I need more help setting up an RIA or ELISA analysis. We provide sample data and a detailed example of how to interpolate unknowns from RIA or ELISA standard ...

GraphPad FAQs

1. Create the data table From the Welcome or New Table dialog, choose to create an XY data table, and select the sample data set: RIA or ELISA.

https://www.graphpad.com/guides/prism/latest/curve-fitting/

From the Welcome dialog, choose the XY tab, drop the list of sample data sets and choose "RIA or ELISA". · Note that the X values are logarithms of concentration ...

GraphPad FAQs

For the four-parameter fit, choose the "Sigmoidal dose-response (variable slope)" model. Step by step examples: RIA/ELISA Dose response curve. If you use Prism ...

GraphPad FAQs

For an example of how to proceed with nonlinear data, see the example on Analyzing RIA or ELISA Data. In the Parameters: Linear regression dialog box, check ...

GraphPad FAQs

Enter the standard curve (also inown as a calibration curve) in the top rows of a table, and then enter the unknown values right below those standards.

https://www.graphpad.com/guides/prism/latest/curve-fitting/

The EC50 is defined quite simply as the concentration of agonist that provokes a response halfway between the baseline (Bottom) and maximum response (Top).

https://www.graphpad.com/guides/prism/latest/curve-fitting/

The goal is to determine the Kd (ligand concentration that binds to half the receptor sites at equilibrium) and Bmax (maximum number of binding sites).

https://www.graphpad.com/guides/prism/latest/curve-fitting/

RIA and ELISA · User-defined equations · Family of theoretical curves · Repeated experiments · Prism scripts. Explore the Knowledgebase. Analyze, graph and ...

GraphPad FAQs