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Prism 3 -- Analyzing RIA and ELISA Data · Enter the Data. In the Welcome to Prism dialog box, select Create a new project and Work independently. · Transform ...

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From the Welcome or New Table dialog, choose to create an XY data table, and select the sample data set: RIA or ELISA. Interpolate unknowns from sigmoidal curve ...

https://www.graphpad.com/guides/prism/latest/curve-fitting/

I need more help setting up an RIA or ELISA analysis. We provide sample data and a detailed example of how to interpolate unknowns from RIA or ELISA standard ...

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For the four-parameter fit, choose the "Sigmoidal dose-response (variable slope)" model. Step by step examples: RIA/ELISA Dose response curve. If you use Prism ...

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From the Welcome dialog, choose the XY tab, drop the list of sample data sets and choose "RIA or ELISA". · Note that the X values are logarithms of concentration ...

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For an example of how to proceed with nonlinear data, see the example on Analyzing RIA or ELISA Data. In the Parameters: Linear regression dialog box, check ...

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Enter the standard curve (also inown as a calibration curve) in the top rows of a table, and then enter the unknown values right below those standards.

https://www.graphpad.com/guides/prism/latest/curve-fitting/

The EC50 is defined quite simply as the concentration of agonist that provokes a response halfway between the baseline (Bottom) and maximum response (Top).

https://www.graphpad.com/guides/prism/latest/curve-fitting/

The goal is to determine the Kd (ligand concentration that binds to half the receptor sites at equilibrium) and Bmax (maximum number of binding sites).

https://www.graphpad.com/guides/prism/latest/curve-fitting/

Problem: In your step-by Step Example: Analyzing RIA and ELISA Data using Prism 3. I followed the steps in your instruction booklet. Six standard points are ...

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